An optimized hair trap for non-invasive genetic studies of small cryptic mammals
作者： Tobias Erik Reiners , Jorge A. Encarnação , Volkmar Wolters
DOI: 10.1007/S10344-011-0543-9
关键词:
摘要: As sample quality and quantity is a crucial factor in non-invasive genetics, we focused on the improvement of sampling efficiency glue hair traps. We invented an optimized trap with moveable parts which enhanced high-quality genetic material. With aid trap, were able to remotely pluck sufficient amount bulbs from our study animal common hamster (Cricetus cricetus) trapping success 49.3% after one survey night. The number collected hairs ranged between 1 50, average 20.7 ± 14.8. Subsequently, use combination simplified laboratory routine allowed us amplify species-specific microsatellites amplification 96.2% ADO 4.6%. This may find usage for species identification or could be used as instrument long-term monitoring mammal populations.
springer.com
sci-hub.se 参考文章(20)
M S Waterman, N Arnheim, W Navidi, A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations. American Journal of Human Genetics. ,vol. 50, pp. 347- 359 ,(1992)
Aritz Ruiz-González, Jonathan Rubines, Oskar Berdión, Benjamín J. Gómez-Moliner, A non-invasive genetic method to identify the sympatric mustelids pine marten (Martes martes) and stone marten (Martes foina): preliminary distribution survey on the northern Iberian Peninsula European Journal of Wildlife Research. ,vol. 54, pp. 253- 261 ,(2008) , 10.1007/S10344-007-0138-7
Cornelia Ebert, Ditmar Huckschlag, Holger K. Schulz, Ulf Hohmann, Can hair traps sample wild boar (Sus scrofa) randomly for the purpose of non-invasive population estimation? European Journal of Wildlife Research. ,vol. 56, pp. 583- 590 ,(2010) , 10.1007/S10344-009-0351-7
M. A. Sloane, P. Sunnucks, D. Alpers, L. B. Beheregaray, A. C. Taylor, Highly reliable genetic identification of individual northern hairy‐nosed wombats from single remotely collected hairs: a feasible censusing method Molecular Ecology. ,vol. 9, pp. 1233- 1240 ,(2000) , 10.1046/J.1365-294X.2000.00993.X
SABINE S. JAKOB, KERSTIN MAMMEN, Eight new polymorphic microsatellite loci for genetic analyses in the endangered common hamster (Cricetus cricetus L.) Molecular Ecology Notes. ,vol. 6, pp. 511- 513 ,(2006) , 10.1111/J.1471-8286.2006.01301.X
Pierre Taberlet, Lisette P Waits, Gordon Luikart, None, Noninvasive genetic sampling: look before you leap Trends in Ecology and Evolution. ,vol. 14, pp. 323- 327 ,(1999) , 10.1016/S0169-5347(99)01637-7
X. Valderrama, W. B. Karesh, D. E. Wildman, D. J. Melnick, Noninvasive methods for collecting fresh hair tissue Molecular Ecology. ,vol. 8, pp. 1749- 1750 ,(1999) , 10.1046/J.1365-294X.1999.00738.X
Thomas Broquet, Nelly Ménard, Eric Petit, Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates Conservation Genetics. ,vol. 8, pp. 249- 260 ,(2006) , 10.1007/S10592-006-9146-5
Glenn M. Sanecki, Ken Green, A technique for using hair tubes beneath the snowpack to detect winter-active small mammals in the subnivean space European Journal of Wildlife Research. ,vol. 51, pp. 41- 47 ,(2005) , 10.1007/S10344-004-0069-5
A. Mortelliti, L. Boitani, Inferring red squirrel (Sciurus vulgaris) absence with hair tubes surveys: a sampling protocol European Journal of Wildlife Research. ,vol. 54, pp. 353- 356 ,(2008) , 10.1007/S10344-007-0135-X