On the study of microbial transcriptomes using second- and third-generation sequencing technologies.
作者： Sang Chul Choi
DOI: 10.1007/S12275-016-6233-2
关键词:
摘要: Second-generation sequencing technologies transformed the study of microbial transcriptomes. They helped reveal transcription start sites and antisense transcripts species, improving genome annotation. Quantification genome-wide gene expression levels allowed for functional studies research. Ever-evolving are reshaping approaches to studying Recently, Oxford Nanopore Technologies delivered a platform called MinION, third-generation technology, research community. We expect it be next technology that enables breakthroughs in life science fields. The transcriptomes will no exception. In this paper, we review transcriptomics using second- generation technology. also discuss prospect with thirdgeneration sequencing.
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