Molecular requirements for the mu-induced light chain gene rearrangement in pre-B cells.

摘要: During B cell differentiation rearrangement of immunoglobulin (Ig) genes is partially regulated by the Ig proteins. Rearrangement heavy (H) chain inhibited, whilst that light (L) induced membrane form mu H chain. In order to analyse additional structural requirements L gene we transfected wild-type and mutant constructs lacking functional exons encoding first or second constant domains into Abelson murine leukemia virus (AMuLV) transformed pre-B cells. All chains are expressed on surface all associate with omega iota, two proteins forming a surrogate chain, necessary for expression. Nevertheless, only not promote rearrangement. A heterodimer Mr 33 kd 36 was found associated but Continuous presence required recombination since loss stopped readdition started We propose protein complex composed kd/36 responsible activation locus its