Trace determination of urinary 3-hydroxybenzo[a]pyrene by automated column-switching high-performance liquid chromatography.

摘要: 3-Hydroxybenzo[a]pyrene (3-OHB[a]P), one of the metabolites benzo[a]pyrene (B[a]P), has been determined in human urine using an automated column-switching procedure. The hydrolysed biological sample is centrifuged just prior to being injected into a reusable precolumn loop, which packed with preparative phase and coupled on-line liquid chromatographic (LC) system. A rapid pre-treatment sample, consisting concentration crude clean-up, performed on precolumn. analytes are then non-selectively desorbed LC eluent cleaned again three successive purification columns direct transfer or "heart-cut" technique. does not exceed 3 min. entire analytical separation procedure takes less than 30 Average 3-OHB[a]P recovery reaches 95% 1-50 ng/l range urine, detection limit 0.1 for ml injection urine. developed method was compared more time-consuming off-line analyse urines B[a]P gavaged rats; statistical treatment indicates that both methods agreement. applied purify concentrate samples workers exposed apparently unexposed polycyclic aromatic hydrocarbons (PAHs).