Comparison of the kinetics of various biomarkers of benzo[a]pyrene exposure following different routes of entry in rats.

摘要: The effect of route exposure on the kinetics key biomarkers to benzo[a]pyrene (BaP), a known human carcinogen, was studied. Rats were exposed an intravenous, intratracheal, oral and cutaneous dose 40 µmol kg–1 BaP. BaP several metabolites measured in blood, urine feces collected at frequent intervals over 72 h post-treatment, using high-performance liquid chromatography/fluorescence. Only 3-hydroxyBaP (3-OHBaP) detectable blood all time points. There route-to-route differences excreted amounts (% dose) but observed courses excretion rate quite similar. In urine, total 0–72 h period followed order: trans-4,5-dihydrodiolBaP (4,5-diolBaP) ≥ 3-OHBaP > 7-OHBaP ≥ 7,8-diolBaP after intravenous injection intratracheal instillation; 3-OHBaP ≈ 7-OHBaP ≥ 4,5-diolBaP > 7,8-diolBaP application; 3-OHBaP ≥ 4,5-diolBaP ≈ 7-OHBaP > 7,8-diolBaP following administration. feces, recovered were: 7-OHBaP ≈ 3-OHBaP > 4,5-diolBaP > 7,8-diolBaP > BaP-7,8,9,10-tetrol administration routes. For routes, 4,5- 7,8-diolBaP almost complete 0–24 h contrast with that 3- 7-OHBaP. This study confirms interest measuring multiple due relative different diolBaPs versus OHBaPs. Concentration ratios may help indicate main exposure. Copyright © 2014 John Wiley & Sons, Ltd.