Functional analysis of the Volvox carteri asymmetric division protein GlsA

摘要: The Zuotin-family J protein chaperone GlsA is essential for the asymmetric divisions that establish germ and somatic cell initials during embryogenesis in green alga Volvox carteri, but it not known on what cellular process acts to carry out this function. Most nuclear, possesses two SANT domains, suggesting may function as a transcriptional regulator. On other hand, close homologs from yeast mice are ribosome-associated factors regulate translation fidelity, implying might also translation. Here we set gain additional evidence regarding of GlsA, specifically with respect its possible involvement transcription We found like zuotin mutants, glsA mutants ultrasensitive both cold ribosome-binding aminoglycoside antibiotic paromomycin, so some fraction likely be ribosome associated. co-immunoprecipitates histones interaction dependent presence intact domains. Through rescue experiments using transgenes encode variants, determined growth division defects mutant separable-a variant rescued did completely phenotype. Considered total, our results suggest at level transcription, tolerance paromomycin sufficient division.