Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici

作者: Sudhamoy Mandal , Adinpunya Mitra , Nirupama Mallick

DOI: 10.1016/J.PMPP.2008.04.002

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摘要: Abstract The oxidative burst or rapid and transient production of large amount reactive oxygen species (ROS) belongs to the fastest earliest active defense responses microbial infection known in plants. aim this study was investigate intensity timing ROS formation, lipid peroxidation expression antioxidant enzymes as initial tomato ( Solanum lycopersicum L.) against invading necrotrophic pathogen Fusarium oxysporum f. sp. lycopersici . concentration hydrogen peroxide (H 2 O ) 2.6 times higher at 24 h post-inoculation (hpi) 4.4 72 hpi extracts inoculated roots than control. An increase total phenolic content also detected roots. activities antioxidative enzymes, viz., superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, 1.11.1.6), guaiacol peroxidase (GPX, 1.11.1.7) ascorbate (APX, 1.11.1.11), increased response inoculation. SOD activity 48 hpi 2.9 that CAT showed a decrease after 24 hpi GPX APX insignificant generated interaction between F. may be an early first line by host mounted pathogen. However, seemingly less efficient system (particularly 24 hpi) leading sustained accumulation observed rate indicate biochemical events are largely favour pathogen, thus making host–pathogen compatible combination. It is discussed served weapon for because not strong enough impede ingress host.

参考文章(50)
Bob Asselbergh, Katrien Curvers, Soraya C. França, Kris Audenaert, Marnik Vuylsteke, Frank Van Breusegem, Monica Höfte, Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis. Plant Physiology. ,vol. 144, pp. 1863- 1877 ,(2007) , 10.1104/PP.107.099226
Matthew E. Spletzer, Alexander J. Enyedi, Salicylic Acid Induces Resistance to Alternaria solani in Hydroponically Grown Tomato. Phytopathology. ,vol. 89, pp. 722- 727 ,(1999) , 10.1094/PHYTO.1999.89.9.722
R. Tenhaken, A. Levine, L. F. Brisson, R. A. Dixon, C. Lamb, Function of the oxidative burst in hypersensitive disease resistance Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 4158- 4163 ,(1995) , 10.1073/PNAS.92.10.4158
Cornelia Göbel, Ivo Feussner, Sabine Rosahl, Lipid peroxidation during the hypersensitive response in potato in the absence of 9-lipoxygenases. Journal of Biological Chemistry. ,vol. 278, pp. 52834- 52840 ,(2003) , 10.1074/JBC.M310833200
Alex Levine, Raimund Tenhaken, Richard Dixon, Chris Lamb, H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response Cell. ,vol. 79, pp. 583- 593 ,(1994) , 10.1016/0092-8674(94)90544-4