Engineering macroscale cell alignment through coordinated toolpath design using support-assisted 3D bioprinting.

摘要: Aligned cells provide direction-dependent mechanical properties that influence biological and function in native tissues. Alignment techniques such as casting uniaxial stretching cannot fully replicate the complex fibre orientation of tissue heart. In this study, bioprinting is used to direct cell alignment. A 0°-90° grid structure was printed assess robustness support-assisted technique. The variation angles pattern designed mimic differences fibril tissues, where alignment vary across different layers. Through a cell-hydrogel mixture, C2C12 displayed directed along longitudinal axis struts. Cell induced through firstly establishing structurally stable constructs (i.e. distinct structures) secondly, allowing dynamically remodel bioprinted construct. Herein reports method inducing macroscale level controlled with angle variation. This not achievable both terms methods conventional electrical stimulation) magnitude hydrogel features less than 100 µm features).