Electrical stimulation of cardiac adipose tissue-derived progenitor cells modulates cell phenotype and genetic machinery.

摘要: A major challenge of cardiac tissue engineering is directing cells to establish the physiological structure and function myocardium being replaced. Our aim was examine effect electrical stimulation on cardiodifferentiation potential adipose tissue-derived progenitor (cardiac ATDPCs). Three different protocols were tested; selected protocol consisted 2 ms monophasic square-wave pulses 50 mV/cm at 1 Hz over 14 days. Cardiac subcutaneous ATDPCs grown biocompatible patterned surfaces. Cardiomyogenic differentiation examined by real-time PCR immunocytofluorescence. In ATDPCs, MEF2A GATA-4 significantly upregulated day 14 after stimulation, while only exhibited increased Cx43 expression. response elongated, both became aligned following linear surface pattern construct. ATDPC length 11.3%, diminished 11.2% (p = 0.013 p = 0.030 vs unstimulated controls, respectively). Compared electrostimulated better surfaces when perpendicular electric field (89.71 ± 28.47o for 92.15 ± 15.21o Electrical caused changes in cell phenotype genetic machinery, making them more suitable regeneration approaches. Thus, it seems advisable use training before delivery as a suspension or within engineered tissue.