On the Inhibition of Gluconeogenesis by 1-β-Phenyl-Ethylbiguanide in the Perfused Guinea Pig Liver

摘要: Summary Altschuld and Kruger (1967) reported that gluconeogenesis from lactate was inhibited by phenethylbiguanide (DBI) in the perfused guinea pig liver. The authors attributed this effect to a concomitant suppression of ATP/ADP ratio. We found glucose formation pyruvate, alanine fructose also lowered biguanides isolated Under our experimental conditions 2 × 10-5 M DBI reduced about 20%. pattern hepatic metabolites indicated crossover phenomenon between 3-phosphoglycerate glyerinaldehyde-phosphate. pyruvate metabolism affected, however, no clear-cut results were obtained with regard carboxylation. glutamate/2-oxoglutarate NH4 ratio rose almost 300% presence DBI, whereas ATP/ADP-, lactate/pyruvate- 3-hydroxybutyrate/acetoacetate not changed. therefore assume small variations intramitochondrial redox state are probably reflected lactate/pyruvate As citrate 2-oxoglutarate concentrations decreased activity citric acid cycle seemed be affected. Whether observed gluconeogenic pathway can an inhibitory on cell respiration could decided metabolite pattern. A decrease cause phenomenon, it may have played additive role if occurred increasing doses biguanides. influence output dependent time animals had been starved prior liver perfusion hydrogen concentration medium. Alkalinization perfusate increased action DBI.