Genome-Wide Screen for New Components of the Drosophila melanogaster Torso Receptor Tyrosine Kinase Pathway.

摘要: Patterning of the Drosophila embryonic termini by Torso (Tor) receptor pathway has long served as a valuable paradigm for understanding how tyrosine kinase signaling is controlled. However, mechanisms that underpin control Tor remain to be fully understood. In particular, it unclear Perforin-like protein Torso-like (Tsl) localizes activity termini. To shed light on this, together with other aspects function, we conducted genome-wide screen identify new components operate downstream Tsl. Using set molecularly defined chromosomal deficiencies, screened suppressors ligand-dependent induced unrestricted Tsl expression. This approach yielded 59 genomic suppressor regions, 11 which mapped causative gene, and further 29 were twins ( tws ), encodes an integral subunit phosphatase 2A complex, α-tubulin at 84B αTub84B major constituent microtubule network, suggesting these may play important part in terminal patterning. Together, data comprise resource discovery components. Many also required roles development, such larval prothoracic gland where controls initiation metamorphosis.