Enzymatic dissociation of long muscles from mice: a model for the study of skeletal muscle fiber types

摘要: SUMMARY The enzymatic dissociation of short muscles from mice, such as flexor digitorum brevis, has allowed a great accumulation physiological, pharmacological and biochemical knowledge about skeletal muscle. However, this body been restricted to the types fibers present in these muscles. Information other fiber limited primarily obtained by manual isolation species, typically rats, via difficult time-consuming procedure. In report, author describes technique for long muscles, soleus or extensor longus (EDL), which can be applied study wider spectrum larger quantities cells. Additionally, kinetics Ca 2+ transients EDL are compared report. usefulness methodology molecular biology experiments is also discussed. This introduces possibility using whole normal muscle pathophysiology diseases.