Effect of Cell Surface Hydrophobicity, Charge, and Zymolectin Density on the Flocculation of Saccharomyces Cerevisiae1
作者： Yu-Lai Jin , Lisa L. Ritcey , R. Alex Speers , Peter J. Dolphin
关键词:
摘要: Cell surface investigations for the yeast strains LCC1209 (Flol phenotype), LCC125 (NewFlo and LCC1240, a nonflocculent variant of LCC1209, are reported. The were examined their degree flocculation, hydrophobicity, charge. zymolectin density was quantitated with fluorescent avidinfluorescein isothiocyanate probe as well radio-labeled tritiated-mannose probe. Additionally, fluorescence microscopy employed to examine cell walls two flocculent strains. showed different charges, apparent densities. hydrophobicity correlated flocculation significantly (r = 0.684, P < 0.001). It found that 82 75% variations in (n 48) can be explained by level affinity Flol NewFlo strains, respectively.
sci-hub.se 参考文章(28)
Malcolm Stratford, Yeast flocculation: receptor definition by mnn mutants and concanavalin A. Yeast. ,vol. 8, pp. 635- 645 ,(1992) , 10.1002/YEA.320080807
B L Miki, N H Poon, V L Seligy, Repression and induction of flocculation interactions in Saccharomyces cerevisiae. Journal of Bacteriology. ,vol. 150, pp. 890- 899 ,(1982) , 10.1128/JB.150.2.890-899.1982
B L Miki, N H Poon, A P James, V L Seligy, Possible mechanism for flocculation interactions governed by gene FLO1 in Saccharomyces cerevisiae. Journal of Bacteriology. ,vol. 150, pp. 878- 889 ,(1982) , 10.1128/JB.150.2.878-889.1982
Marika H. Straver, Jan W. Kijne, A rapid and selective assay for measuring cell surface hydrophobicity of brewer's yeast cells Yeast. ,vol. 12, pp. 207- 213 ,(1996) , 10.1002/(SICI)1097-0061(19960315)12:3<207::AID-YEA899>3.0.CO;2-U
Manuel Mota, Eduardo V. Soares, Population dynamics of flocculating yeasts Fems Microbiology Reviews. ,vol. 14, pp. 45- 51 ,(1994) , 10.1111/J.1574-6976.1994.TB00071.X
M. C. Penalver, M. Casanova, J. P. Martinez, M. L. Gil, Cell wall protein and glycoprotein constituents of Aspergillus fumigatus that bind to polystyrene may be responsible for the cell surface hydrophobicity of the mycelium Microbiology. ,vol. 142, pp. 1597- 1604 ,(1996) , 10.1099/13500872-142-7-1597
Neil W. Taylor, William L. Orton, ROMATIC COMPOUNDS AND SUGARS IN FLOCCULATION OFSACCHAROMYCES CEREVISIAE Journal of the Institute of Brewing. ,vol. 84, pp. 113- 114 ,(1978) , 10.1002/J.2050-0416.1978.TB03850.X
N. van UDEN, L. DO CARMO SOUSA, Yeasts from the bovine caecum. Microbiology. ,vol. 16, pp. 385- 395 ,(1957) , 10.1099/00221287-16-2-385
Tracy W Perkins, Derek S Mak, Thatcher W Root, Edwin N Lightfoot, Protein retention in hydrophobic interaction chromatography: modeling variation with buffer ionic strength and column hydrophobicity Journal of Chromatography A. ,vol. 766, pp. 1- 14 ,(1997) , 10.1016/S0021-9673(96)00978-8
Marika H. Straver, Vincent M. Traas, Gerrit Smit, Jan W. Kijne, Isolation and partial purification of mannose‐specific agglutinin from brewer's yeast involved in flocculation Yeast. ,vol. 10, pp. 1183- 1193 ,(1994) , 10.1002/YEA.320100906