Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis.
作者： Sai-Yong Zhu , Xiang-Chun Yu , Xiao-Jing Wang , Rui Zhao , Yan Li
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摘要: Many biochemical approaches show functions of calcium-dependent protein kinases (CDPKs) in abscisic acid (ABA) signal transduction, but molecular genetic evidence linking defined CDPK genes with ABA-regulated biological at the whole-plant level has been lacking. Here, we report that ABA stimulated two homologous CDPKs Arabidopsis thaliana, CPK4 and CPK11. Loss-of-function mutations CPK11 resulted pleiotropic ABA-insensitive phenotypes seed germination, seedling growth, stomatal movement led to salt insensitivity germination decreased tolerance seedlings stress. Double mutants had stronger ABA- salt-responsive than single mutants. CPK4- or CPK11-overexpressing plants generally showed inverse ABA-related relative those loss-of-function Expression levels many ABA-responsive were altered overexpression lines. The both phosphorylated transcription factors, ABF1 ABF4, vitro, suggesting may regulate signaling through these factors. These data provide planta for involvement CDPK/calcium are important positive regulators CDPK/calcium-mediated pathways.
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plantcell.org参考文章(80)
Z. M. Pei, J. M. Ward, J. F. Harper, J. I. Schroeder, A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK. The EMBO Journal. ,vol. 15, pp. 6564- 6574 ,(1996) , 10.1002/J.1460-2075.1996.TB01047.X
R. W. McMichael Jr, M. Bachmann, S. C. Huber, Spinach Leaf Sucrose-Phosphate Synthase and Nitrate Reductase Are Phosphorylated/Inactivated by Multiple Protein Kinases in Vitro Plant Physiology. ,vol. 108, pp. 1077- 1082 ,(1995) , 10.1104/PP.108.3.1077
M. Bachmann, R. W. McMichael Jr, J. L. Huber, W. M. Kaiser, S. C. Huber, Partial Purification and Characterization of a Calcium-Dependent Protein Kinase and an Inhibitor Protein Required for Inactivation of Spinach Leaf Nitrate Reductase Plant Physiology. ,vol. 108, pp. 1083- 1091 ,(1995) , 10.1104/PP.108.3.1083
Sylvain Merlot, Françoise Gosti, Danièle Guerrier, Alain Vavasseur, Jérôme Giraudat, The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant Journal. ,vol. 25, pp. 295- 303 ,(2001) , 10.1046/J.1365-313X.2001.00965.X
Miguel A. Rodriguez Milla, Jared Townsend, Ing-Feng Chang, John C. Cushman, The Arabidopsis AtDi19 Gene Family Encodes a Novel Type of Cys2/His2 Zinc-finger Protein Implicated in ABA-independent Dehydration, High-salinity Stress and Light Signaling Pathways Plant Molecular Biology. ,vol. 61, pp. 13- 30 ,(2006) , 10.1007/S11103-005-5798-7
Liu-Min Fan, Zhixin Zhao, Sarah M Assmann, Guard cells: a dynamic signaling model Current Opinion in Plant Biology. ,vol. 7, pp. 537- 546 ,(2004) , 10.1016/J.PBI.2004.07.009
Riichiro Yoshida, Taishi Umezawa, Tsuyoshi Mizoguchi, Seiji Takahashi, Fuminori Takahashi, Kazuo Shinozaki, The Regulatory Domain of SRK2E/OST1/SnRK2.6 Interacts with ABI1 and Integrates Abscisic Acid (ABA) and Osmotic Stress Signals Controlling Stomatal Closure inArabidopsis Journal of Biological Chemistry. ,vol. 281, pp. 5310- 5318 ,(2006) , 10.1074/JBC.M509820200
Jiahong Shao, Alice C. Harmon, In vivo phosphorylation of a recombinant peptide substrate of CDPK suggests involvement of CDPK in plant stress responses. Plant Molecular Biology. ,vol. 53, pp. 691- 700 ,(2003) , 10.1023/B:PLAN.0000019112.37316.C8
L. Lopez-Molina, S. Mongrand, N.-H. Chua, A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 4782- 4787 ,(2001) , 10.1073/PNAS.081594298
J. Sheen, Ca2+-Dependent Protein Kinases and Stress Signal Transduction in Plants Science. ,vol. 274, pp. 1900- 1902 ,(1996) , 10.1126/SCIENCE.274.5294.1900