Metals in Cells: X‐Ray Fluorescence Microscopy
作者： James E. Penner-Hahn
DOI: 10.1002/9781119951438.EIBC2110
关键词:
摘要: X-ray fluorescence is one of the few tools that can be used to determine concentration and localization metals nonmetals in cells. Depending on element experimental conditions, typically provides modest sensitivity, micromolar range (i.e., high parts per billion sensitivity), superb mass detection limits, as low attomoles. Spatial resolution ranges from micrometers tens nanometers. In addition basic quantification, also offers possibility defining chemical speciation an based changes its absorption near-edge structure. This article reviews physical principles microscopy summarizes applications biological samples, including a discussion strengths weaknesses comparison other analytical methods. Keywords: X-ray fluorescence; synchrotron; XANES; microprobe; nanoprobe; PIXE; metallome
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