A comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris : temperature effect and scale-up simulation

摘要: The production of recombinant proteins by Pichia pastoris under AOX1 promoter is usually performed using methanol together with either glycerol or sorbitol as co-substrate. Although both co-substrates have been widely used, comparative studies are scarce. In addition, these comparisons at different specific growth rate (µ) that it well known has an important effect on productivity. Thus, the Rhyzopus oryzae lipase (ROL) P. was compared in continuous cultures growing same µ 22 30 °C. Results show co-substrate led to higher volumetric productivities, and lower consumption rates. Scale-up simulation 10–10,000 L bioreactor sizes indicated produced highest productivity ROL aeration requirements. Therefore, rises a better option than production.