Dynamic Chromatin Modification Sustains Epithelial-Mesenchymal Transition following Inducible Expression of Snail-1
作者： Sarah Javaid , Jianmin Zhang , Endre Anderssen , Josh C. Black , Ben S. Wittner
DOI: 10.1016/J.CELREP.2013.11.034
关键词:
摘要: Summary Epithelial-mesenchymal transition (EMT) is thought to contribute cancer metastasis, but its underlying mechanisms are not well understood. To define early steps in this cellular transformation, we analyzed human mammary epithelial cells with tightly regulated expression of Snail-1, a master regulator EMT. After Snail-1 induction, markers were repressed within 6 hr, and mesenchymal genes induced at 24 hr. binding target promoters was transient (6–48 hr) despite continued protein expression, it followed by both long-lasting chromatin changes. Pharmacological inhibition selected histone acetylation demethylation pathways suppressed the induction as maintenance Snail-1-mediated Thus, EMT involves an epigenetic switch that may be prevented or reversed use small-molecule inhibitors modifiers.
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