Is a collagen scaffold for a tissue engineered nucleus replacement capable of restoring disc height and stability in an animal model?

摘要: The idea of a tissue engineered nucleus implant is to seed cells in three-dimensional collagen matrix. This matrix may serve as scaffold for implant. aim this study was investigate whether implantation the into spinal segment after nucleotomy able restore disc height and flexibility. basically consists condensed type-I For clinical use, will be used reinforcing supporting culturing cells. In experiments, matrixes were concentrated with barium sulfate X-ray purposes cell seeding disclaimed order evaluate biomechanical performance material. Six bovine lumbar functional units, aging between 5 6 months, in-vitro test. each specimen, an oblique incision performed, removed replaced by collagen-type-I Specimens mounted custom-built spine tester, subsequently exposed pure moments 7.5 Nm move within three anatomical planes. Each tested stage (intact, implanted) evaluated range motion, neutral zone change height. Removal significantly reduced 0.84 mm respect intact caused instability segment. Through it possible stability loss, even increase slightly 0.07 compared stage. There no statistical difference provided Results movements lateral bending axial rotation showed same trend flexion/extension. However, extrusions have been observed six cases during flexibility assessment. results directly reflect efficacy vital replacement provide intervertebral discs. from point view, challenge employ appropriate annulus fibrosus sealing method, which capable keep place over long-time period. Securing inside one most important prerequisites if such shall chance application.