Ginsenoside Rg3 Induces Browning of 3T3-L1 Adipocytes by Activating AMPK Signaling.
作者： Kyungtae Kim , Ki Hong Nam , Sang Ah Yi , Jong Woo Park , Jeung-Whan Han
DOI: 10.3390/NU12020427
关键词:
摘要: Ginsenoside Rg3, one of the major components in Panax ginseng, has been reported to possess several therapeutic effects including anti-obesity properties. However, its effect on browning mature white adipocytes as well underlying mechanism remains poorly understood. In this study, we suggested a novel role Rg3 3T3-L1 by upregulating browning-related gene expression. The differentiated were evaluated analyzing markers using quantitative PCR, immunoblotting, and immunostaining. addition, size sum area lipid droplets measured Oil-Red-O staining. adipocytes, dose-dependently induced expression genes such Ucp1, Prdm16, Pgc1α, Cidea, Dio2. Moreover, beige fat-specific (CD137 TMEM26) metabolism-associated (FASN, SREBP1, MCAD), which indicated activation metabolism Rg3. We also demonstrated that 5' adenosine monophosphate-activated protein kinase (AMPK) is required for Rg3-mediated up-regulation inhibited accumulation reduced droplet adipocytes. Taken together, study identifies suggesting potential an ginseng.
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