Investigation on the Interaction between Cyclophosphamide and Lysozyme in the Presence of Three Different Kind of Cyclodextrins: Determination of the Binding Mechanism by Spectroscopic and Molecular Modeling Techniques
作者： Mahdieh Mansouri , Malihe Pirouzi , Mohammad Saberi , Maryam Ghaderabad , Jamshidkhan Chamani
DOI: 10.3390/MOLECULES18010789
关键词:
摘要: The interactions between cyclophosphamide (CYC) and lysozyme (LYZ) in the presence of different cyclodextrins (CDs) were investigated by UV absorption, fluorescence spectroscopy, circular dichroism (CD), molecular modeling techniques under imitated physiological conditions. absorption results showed formation complexes CYC LYZ CDs. Fluorescence data show that has a stronger quenching effect on LYZ, red shifts suggested microenvironment Trp residues was changed became more hydrophilic. interaction with properties caused strong static binary ternary systems. binding affinities as well number sites obtained from CDs systems modified Stern-Volmer plots. Resonance Light Scattering (RLS) technique utilized to investigate drug conformational changes separate simultaneous. enhancement RLS intensity attributed complex protein absence conformation analyzed using synchronous spectroscopy. Our revealed originated Tyr residues, demonstrated addition distances donor acceptor (CYC CDs) estimated according Forster’s theory for CD spectra indicated induced secondary structural Molecular differ those distance confirmed experimental results.
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