Mass Spectrometry of Biological Molecules

摘要: Mass spectrometric methods may serve as powerful analytical in life sciences and medicine. spectrometry (MS) is comparable sensitivity to techniques based on chromatography or electrophoresis but has superior resolution. Although there are a number of suitable ionization methods, allowing the analysis biological molecules that high molecular weight low volatility, mainly two “soft-ionization” confer only minimum degradation analyte: MALDI (matrix-assisted laser desorption/ionization) ESI (electrospray ionization). The following article provides short overview advantages drawbacks samples. Whereas most applications field protein analysis, analyses carbohydrates lipids also comprehensively discussed. It shown useful tool especially lipid whereas with carbohydrate still problems have be solved. rapid very sensitive (from picomolar attomolar range) tolerates amounts impurities (in contrast ESI), which typically present A further advantage singly charged ions formed; this enables applied even crude mixtures although ion yields differ significantly dependence interest. In comparison ESI, detects compounds higher weights more sensitively, for labile compounds. Therefore, combination both is, many cases, ideal solution problems.