Activation of src-related tyrosine kinases by IL-3.

摘要: The activation of src-related tyrosine kinases following IL-3 stimulation was examined in 32Dcl3 cells. Three were activated stimulation: fyn, hck, and lyn. cells transfected with retroviral vectors expressing each these independent clones overexpressing kinase isolated. In either fyn or stimulated a rapid increase catalytic activity, which remained elevated longer compared the kinetics observed parental An number tyrosine-phosphorylated proteins presence absence hck. Transfection vector encoding lyn also resulted an level although not as dramatic that Consistent observations cells, high basal activity unstimulated lyn-transfected approximate threefold activity. Overexpression c-src did result IL-3-stimulated c-src, indicating specificity for While overexpression increased phosphorylation, it render more sensitive to IL-3. These results suggests addition JAK2 kinase, may play significant role signal transduction by cytokine receptors.