Ternary vitronectin-thrombin-antithrombin III complexes in human plasma. Detection and mode of association.
作者： H.C. de Boer , P.G. de Groot , B.N. Bouma , K.T. Preissner
DOI: 10.1016/S0021-9258(18)54071-0
关键词:
摘要: Radiolabeled antithrombin III (ATIII) was incubated at 37 degrees C with purified vitronectin (VN) or fibrinogen-deficient plasma before thrombin added to initiate complex formation. Incorporation of radiolabeled ATIII detected using polyacrylamide gel electrophoresis (PAGE) and autoradiography. The PAGE conditions appeared be crucial for the detection VN.TAT complexes. In absence SDS, ternary complexes formed instantaneously, whereas in presence only 50% TAT associated VN after a 60-min incubation. Formation could confirmed by filtration which added. Furthermore, patient plasmas (disseminated intravascular coagulation sepsis) found bind heparin-Sepharose, indicating that this endogenously also VN. amino-terminal region moiety were responsible their interaction, stabilized disulfide bridges. These results indicate normal all is complexed This association alters conformational state VN, appears clearance from circulation.
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