Dynamics of Oscillating Erythrocyte Doublets after Electrofusion

摘要: Abstract Erythrocytes were electrofused with multiple rectangular voltage pulses to show an oscillatory movement, divided into swell phases and pump events. During each phase, which lasted from 0.5s more than 180s, the fused cells' (doublets') volume increased by colloid osmotic swelling, membrane area was expanded until rupture. Membrane rupture initiated event, where doublets' decreased almost exponential time course constants between 2ms 8ms. Simultaneously, a portion of cytosolic hemoglobin solution ejected extracellular space ("jet"). Pump event phase durations rising chamber temperature, indicating that both parts movements determined physical properties liquids. Relative change developments express gradual loss elasticity during oscillation, decreasing elastic forces stored in membrane. Evidence is given first causes weakening at site. Heat treatment up 45°C had negligible effect on times, constants, relative changes. A heat 50°C prevented movements. The location accorded theories potential induced electropermeabilization.