Sustained release of ascorbate-2-phosphate and dexamethasone from porous PLGA scaffolds for bone tissue engineering using mesenchymal stem cells.
作者： Hyongbum Kim , Hyun Woo Kim , Hwal Suh
DOI: 10.1016/S0142-9612(03)00358-2
关键词:
摘要: The purpose of this research was to develop porous poly(D,L-lactide-co-glycolide) (PLGA) scaffolds from which ascorbate-2-phosphate (AsAP) and dexamethasone (Dex) are continuously released for a month osteogenesis mesenchymal stem cells bone tissue engineering. Porous PLGA matrices containing AsAP Dex were prepared by solvent casting/particulate leaching method. In vitro release water uptake studies performed in Dulbecco's phosphate buffered saline at 37 degrees C 15 rpm. Drug loading rates determined high performance liquid chromatography. Release showed that, after an initial burst lasting 4 9 days, respectively, followed zero order kinetics with correlation coefficients least until 35 days. Incorporation into the increased AsAP, scaffold uptake. When (MSCs) cultured vitro, amount mineralization significantly higher than control scaffolds. conclusion, incorporated over MSCs culture these
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