Biological activities of the hydro-alcoholic and aqueous extracts of Achillea fragrantissima (Forssk.) grown in Jordan

摘要: Aqueous and hydro-alcoholic extracts of Achillea fragrantissima L. (Asteraceae) grown in Jordan were screened for their antioxidant, antimicrobial, antiplatelet, anti-proliferative acetylcholinesterase (AChE) inhibition efficacy. Total phenols flavonoids determined colorimetrically. The radical scavenging activities evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity assays. High performance liquid chromatography-mass spectrometry (HPLCMS) analysis resulted the identification 7 phenolic compounds the extract 4 compounds aqueous extract; quercetin 3-β-D-glucoside was main component for both extracts. Antimicrobial by antimicrobial susceptibility testing such as agar well-diffusion method, minimum inhibition concentration minimum bactericidal concentration. Gram positive bacteria showed sensitivity to agar-well diffusion test. No significant observed against gram negative bacteria Candida albicans. Hydro-alcoholic had a Streptococcus pneumoniae Bacillus cereus at high concentrations (MIC 12.5 mg/ml) rather than inhibitory effect. In vitro antiplatelet was tested on human whole blood an electrical impedance method. At concentrations (50, 100, 200 μg/ml), no effect platelet aggregation was noticed. Anti-proliferative investigated MTT assay. up μg/ml, did not possess cytotoxic activity against MCF-7 cells. Acetylcholinesterase (AChE) capacity A. fragrantissima tested using TLC assay neither aqueous, nor hydroalcoholic AChE inhibition. present investigation supported the traditional use Jordanian folk medicine as an antimicrobial active representative genus Achillea. should be further studied their potential preventing/treating diseases which oxidative stress is part pathophysiology.