Isolation of integral membrane proteins of Leishmania promastigotes and evaluation of their prophylactic potential in hamsters against experimental visceral leishmaniasis.

摘要: The integral membrane proteins (IMP's) of promastigotes two virulent strain Leishmania (L.) donovani Dd8 and infantum LV9 one avirulent viscerotropic tropica UR6 were extracted by phase separation technique using a non-ionic detergent "Triton X-114". This is homogeneous at 0 degrees C but divides in an aqueous above 20 C. partition resulted solubilisation hydrophilic phase, IMP's with amphiphilic nature recovered the phase. wise quantitative recovery rates estimated. These purified chill methanol centrifugation used as vaccinogen, alone or combination adjuvant against L. challenge hamster model. Among all combinations, hamsters immunised IMP (Dd8) CFA 75% parasite inhibition spleen (P <0.001), however, 61.14% <0.001) 77.60% parasitic was achieved liver bone marrow respectively compared to their unvaccinated counter part. Similar combinations inhibited establishment up 65.12% 66.87% on splenic site. specific IgG level (Dd8 strain) soluble leishmania promastigote antigen monitored different stages enzyme linked immunosorbent assay (ELISA) corresponds establishment. observations made cases strains. However, significant lymphoproliferative response IMPs (SI 3.5-4.9, P noticed + vaccinated animals. Thus, this study will provide lead for more manipulative trials develop subunit vaccine fatal disease.