Leishmania donovani: Identification of stimulatory soluble antigenic proteins using cured human and hamster lymphocytes for their prophylactic potential against visceral leishmaniasis

摘要: Abstract Most of the studies for identification prophylactic antigens that elicit T cell responses were concentrated on membrane proteins Leishmania donovani . This study was taken up to assess L. soluble promastigote their ability stimulate proliferation peripheral blood mononuclear cells (PBMCs) from cured visceral leishmaniasis (VL) patients, endemic and non-endemic controls lymphocytes/peritoneal macrophages hamsters. The protein subjected sequential precipitation with saturated ammonium sulphate (20%, 40%, 60% 80%), which largely 80% fractioned showed significant cellular in patients fraction further fractionated into five sub fractions by preparative SDS–PAGE re-evaluation induce responses. Out these, only F2 belonging MW 97.4–68 kDa stimulated remarkable lymphoproliferative IFN-γ VL controls. Similarly, nitric oxide production also noticed infected animals indicating an element uniformity between hamster human. fraction, when evaluated its efficacy BCG against challenge exhibited parasite inhibition spleen (71.1%; p