Isolation and characterization of transposon Tn4001-generated, cytadherence-deficient transformants of Mycoplasma pneumoniae and Mycoplasma genitalium

摘要: Cytadherence and subsequent parasitism of host cells by the human pathogens, Mycoplasma pneumoniae genitalium, are mediated adhesins adherence-related accessory proteins. In this report we demonstrate use transposon Tn4001 to generate Tn-induced transformants displaying cytadherence-deficient characteristics. Tn-generated transformant, designated 8R, lacked high-molecular weight adherence-accessory proteins HMW1/4 was deficient in hemadsorption cytadherence capabilities. transformant not localized or near hmw1 gene upstream adhesin (p30/hmw3) locus, suggesting an alternate site associated with regulation expression. Sequence analysis identified insertion at crl locus previously reported, although protein characteristics 8R differed from earlier described transformants. The M. genitalium Tn-transformant, G26, also defective cytadherence. However, G26 synthesized P140 P32 that transposed into a new unlinked cytadherence, namely ORF MG032. This study demonstrates utility mutagenesis for both which, latter case, has special relevance light recent complete characterization its continuous total genomic sequence.