Binding and Internalization of a Luteinizing Hormone-Releasing Hormone Agonist by Rat Gonadotrophic Cells. A Radioautographic Study
作者： G. PELLETIER , D. DUBE , J. GUY , C. SEGUIN , F. A. LEFEBVRE
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摘要: In order to localize the binding sites for LHRH and possible internalization of peptide in rat pituitary gland, radioautography was performed as a function time after iv injection iodinated stable agonist [DSer(tBu)6,des-Gly-NH2 10]LHRH ethylamide into intact (both sexes) castrated female rats. At light microscopic level, silver grains were found small percentage cells (gonadotrophs) rats restricted castration gonadectomized control animals injected with both [D-Ser(tBu) 6,des-Gly-NH2 an excess unlabeled peptide, no could be detected. electron only gonadotrophs appear contain grains. The time-course study showed that 3 min most associated plasma membrane. longer intervals (10 30 min), label mostly detected Golgi apparatus and, less...
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