[44] Localization of gonadotropin-releasing hormone receptors

摘要: Publisher Summary Gonadotropin-releasing hormone (GnRH) is a decapeptide that binds to receptors on the pituitary gonadotrope and specific cells in gonad. Labels, such as colloidal gold or ferritin provide discrete localization but may compromise potency of hormone. Radioactive labels allow full potency; however, resolution signal after autoradiography does not accurate marking identification smallest organelles pathway. An avidin–biotin labeling system has protocols maintains GnRH analog. The analog used for this protocol one which lysine substituted glycine sixth position, thereby making it available conjugation derivative biotin reacts with amino groups. biotinylated seen black patches cellular process. Quantitative analyses have shown 90% all gonadotropes (containing LH FSH antigens) exhibit reaction GnRH. With avidin–fluorescein protocol, no more than 12 coverslips are stained at time. Tests binding also conducted detection system.