Expression of adhesion molecules is specific and time-dependent in cytokine-stimulated endothelial cells in culture

摘要: The time course of expression the adhesion molecules E-selectin, VCAM-1, ICAM-1 and PECAM-1 was studied in interleukin-1 beta-stimulated human umbilical vein cells (HUVEC) subcellular sites synthesis were determined by means fluorescence immunohistochemistry. maximal number labelled for E-selectin observed at 2-4 h, VCAM-1 4-8 h 6-72 h. At 8 started to disappear, but ICAM-1-positive persisted. constitutively expressed. De novo 1 both, 1.5-2 Maximal synthetic activity 2.5-4 4-6 ICAM-1; thereafter, slowly decreased. Transport granules occurred 1.5 4 VCAM-1; they absent ICAM-1. Diffuse cellular membrane labelling indicative functional began VCAM, present In conclusion, each molecule shows a specific time-dependent appearance disappearance HUVECs accordance with their physiological role vivo. These morphological results confirm data obtained flow cytometry Western blotting, provide new information about behaviour individual regard localization molecules.