Immunohistochemical microquantification of fast-myosin in frozen histological sections of mammalian skeletal muscles

摘要: Fast-myosin in frozen histological sections was quantified by an immunohistochemical micromethod based on the ELISA. Frozen tissue mounted glass slides were used analogously to antigen-precoated wells of ELISA plates. The intensity immunoreactivity anti-fast-myosin monoclonal antibody directly from color developed with second coupled peroxidase using phenol-4-aminoantipyrine as a substrate. levels masseter muscles pigs, rats, and rabbits 185 +/- 6, 223 9, 178 12 mg/g total protein, respectively, those gastrocnemius cows, goats, 172 12, 211 7, 177 10, 205 10 mg/g, respectively. In cows fast-myosin not detected. results obtained this good agreement histomorphometrical biochemical analyses. This could be quantitatively evaluate muscle contractile characteristics that determine meat quality.