A foreign β-globin gene in transgenic mice: Integration at abnormal chromosomal positions and expression in inappropriate tissues
作者： Elizabeth Lacy , Sally Roberts , Edward P. Evans , Mike D. Burtenshaw , Franklin D. Costantini
DOI: 10.1016/0092-8674(83)90369-0
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摘要: Abstract We have investigated the chromosomal location, inheritance, and expression of a cloned rabbit β-globin gene introduced into mouse germ line by microinjection eggs. Experiments utilizing in situ hybridization to metaphase chromosomes show that has integrated one or two different loci each five lines analyzed. Each locus contains between three forty copies foreign DNA sequence arranged tandem array, sequences at are stably inherited as single Mendelian marker. Neither globin mRNA nor polypeptides encoded detected erythroid cells seven transgenic examined, indicating is not correctly regulated. However, lines, transcripts found low level specific, although inappropriate, tissues: skeletal muscle testis another line. These unusual patterns transcription heritable traits may result from gene's integration abnormal positions.
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