Transcriptional control elements and complex initiation pattern of the TATA-less bidirectional human thymidylate synthase promoter.
作者： Shanghong Dong , Laura Lester , Lee F. Johnson
DOI: 10.1002/(SICI)1097-4644(20000401)77:1<50::AID-JCB6>3.0.CO;2-C
关键词:
摘要: The nucleotide sequences that are important for transcription of the human thymidylate synthase gene were analyzed by deletion and site-directed mutagenesis promoter region. Deletion analyses from 5′ 3′ ends indicated presence multiple positive negative elements. had approximately same strength in normal or inverted orientation. region between 161 141 nt upstream translational start codon was found to be both necessary sufficient high-level activity directions designated essential This region, which is highly conserved human, mouse rat TS promoters, contains potential binding sites Ets, Sp1, LSF factors. Site directed each these elements led large decreases strength. However, inactivation Sp1 E2F adjacent increases transcriptional site pattern S1 nuclease protection assays mRNA isolated cells transiently transfected with minigenes. Multiple detected, most 160 120 AUG codon. J. Cell. Biochem. 77:50–64, 2000. © 2000 Wiley-Liss, Inc.
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