Effects of fixation and varying target length on the sensitivity of polymerase chain reaction for detection of human T-cell leukemia virus type I proviral DNA in formalin-fixed tissue sections.

摘要: In this study, the fixation condition most suitable for maintaining sensitivity of polymerase chain reaction (PCR) was investigated by using alpha-tubulin gene sequence, and PCR procedure effective detecting human T-cell leukemia virus type I (HTLV-I) proviral sequences in fixed, embedded tissues adult patients explored. First, targeting a 286-bp sequence studied tissue sections fixed several fixatives various periods at 25 or 4 degrees C. For histological examination, with 10% buffered formalin lower temperature shorter period found to be preferable retain sensitivity. And HTLV-I detected only 7 18 specimens (38.9%) when 374-bp gag region targeted, but rate increased 77.8% (14 specimens) length target reduced 120 bp within same region. Therefore, one-round is application archival specimens, which may not ideal DNA preservation.