Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues.
作者： Renate Schneider , Bernd Gollackner , Bernhard Edel , Katharina Schmid , Friedrich Wrba
DOI: 10.1016/J.IJPARA.2007.11.008
关键词:
摘要: The aim of our study was to establish a new PCR protocol for the detection and discrimination Echinococcus granulosus complex on one hand multilocularis in formalin-fixed, paraffin-embedded tissues (FFPTs) other. target sequences all PCRs are located 471bp segment mitochondrial ND1 gene, fragment sizes amplification products 295bp (for sheep strain E. granulosus), 204bp pig granulosus) 252bp multilocularis), respectively. In total, 80 FFPTs from patients with histologically confirmed echinococcosis (76 four multilocularis) operated Austrian hospitals between 1978 2005 were examined. 68 (85%) samples, we able detect specific DNA fragments newly established protocols. Thirty-eight (47.5%) clinical samples identified as G1 strain, 26 (32.5%) G5, 6 or 7 strains (5%) multilocularis. specificity three 100%; G6 G7 strains, sequencing an additional 234bp necessary showed that out PCR-positive infected genotype (the camel strain).
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