Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain

摘要: Abstract After boiling and extraction with 4M Guanidine HCl to inactive endogenous peptidases, the anterior neurointermediate lobes of rat pituitary were analysed by gel filtration a beta-endorphin radioimmunoassay which detects proopiocortin, beta-lipotropin equimolar sensitivity. In lobe, immunoreactive (ir) was eluted in three peaks: 10% total immunoreactivity had an apparent M r 57% 27% beta-endorphin. all is one peak synthetic Total Beta-endorphin levels lobe increased adrenalectomy decreased after long term administration dexamethasone (0.2 mg/kg for 16 days) or stress (1 hour inescapable footshock). Levels unchanged these treatments. Neither at 0.2 mg/kg/day days any effects on Met 5 -enkephalin Leu striatum, hypothalamus lobe. larger doses mg/kg/each 12 hrs 7 enkephalin striatum (150% controls) but not These data further support independent avenues post-synthetic peptide processing brain lobes.