Similarity Analysis of DNAs

摘要: In contrast to gram-negative bacteria, nearly all gram-positive bacteria must be digested with a lytic enzyme before they can lysed by detergent. addition lysozyme, which is the most commonly used, several other enzymes are available. These include N-acetylmuramidase, isolated from Streptomyces globisporus and also cleaves muramic acid backbone; lysostaphin, an endopeptidase that Staphylococcus sp. strain K-6-WI specific for cross-linking peptides of staphylococci. There two approaches available disrupting recalcitrant bacteria. The first involves making cells susceptible one or more growing them in presence wall-component analogs antibiotics, second use any physical methods cell disruption. moles percent G+C content DNA estimated different methods. described this chapter thermal denaturation, high-performance liquid chromatography (HPLC), dye-binding fluorimetry. Bacteriologists have been criticized being bit sloppy doing reporting reassociation experiments, compared those researchers working eucaryotes. Although some criticism justified, there additional reasons greater variability bacterial experiments. First, sheer number bacteriology laboratories involved performing experiments has contributed results. Second, hydroxylapatite (HA) procedure used eucaryotic studies, whereas procedures discussed extensively bacteriologists.