Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake.

作者: M. C. Gutierrez , A. M. Castillo , M. Kamekura , A. Ventosa

DOI: 10.1099/IJS.0.2008/001602-0

关键词:

摘要: A novel extremely halophilic strain, designated XH-65(T), isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65(T) is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum 3.4 NaCl, grows pH 6.0-9.0, growth 7.5. 25-50 degrees C, optimal 37 C. Magnesium not required growth. On basis of 16S rRNA gene sequence analysis, strain shown belong genus Haloterrigena related turkmenica VKM B-1734(T) (98.1 % similarity), saccharevitans AB14(T) (96.9 %), thermotolerans PR5(T) (96.3 limicola AX-7(T) (95.8 %) hispanica FP1(T) (95.7 %). DNA-DNA hybridization revealed relatedness between Htg. B-1734(T). The polar lipid composition presence phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester mannose-2,6-disulfate (1-->2)-glucose glycerol diether (S(2)-DGD). results physiological biochemical tests allowed genotypic phenotypic differentiation six species validly published names. Therefore, represents species, which name salina sp. nov. proposed, type (=CGMCC 1.6203(T) =JCM 13891(T)).

参考文章(40)
John L. Johnson, William B. Whitman, Similarity Analysis of DNAs American Society of Microbiology. pp. 624- 652 ,(2007) , 10.1128/9781555817497.CH26
Kate Wilson, Preparation of Genomic DNA from Bacteria Current protocols in molecular biology. ,vol. 56, ,(2001) , 10.1002/0471142727.MB0204S56
D R Arahal, F E Dewhirst, B J Paster, B E Volcani, A Ventosa, Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Applied and Environmental Microbiology. ,vol. 62, pp. 3779- 3786 ,(1996) , 10.1128/AEM.62.10.3779-3786.1996
J. De Ley, R. Tijtgat, Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 36, pp. 461- 474 ,(1970) , 10.1007/BF02069048
Russell Vreeland, Scott Straight, Jessica Krammes, Kevin Dougherty, William Rosenzweig, Masahiro Kamekura, Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles. ,vol. 6, pp. 445- 452 ,(2002) , 10.1007/S00792-002-0278-3
A. OREN, P. GUREVICH, R. T. GEMMELL, A. TESKE, Halobaculum gomorrense gen. nov., sp. nov., a novel extremely halophilic archaeon from the dead sea International Journal of Systematic and Evolutionary Microbiology. ,vol. 45, pp. 747- 754 ,(1995) , 10.1099/00207713-45-4-747