Estimation of testosterone in human peripheral blood using S35-thiosemicarbazide.

摘要: A double isotope derivative method for the estimation of progesterone in 10 ml peripheral plasma, using 35S-thiosemicarbazide (100 mc/mM) as reagent and 1,2-3H-progesterone (0.8 mμg, 87 μc/μg) a s indicator, is described. Purification achieved by chromatography first formed, bisthiosemicarbazone, silica gel thin layer paper system, hydrolysis to 3-thiosemicarbazone this finally acetylation 2′,4′-diacetate, subsequent on one system. The values given water samples same volume plasma from ovariectomized, adrenalectomized subjects were 0.0020 ±0.0009 (sd) ±0.0002 (se) μg (23 samples) 0.0029 ±0.0013 ±0.006 (5 samples), respectively. These mean therefore nonspecific blank method. No known naturally occurring steroids found interfere with t...