Comparison of macrophage antimicrobial responses induced by type II interferons of the goldfish (Carassius auratus L.)

摘要: Unlike mammals, bony fish have two type II interferons, IFNγ and IFNγrel, whose pro-inflammatory functions not been fully characterized. To elucidate the distinct roles of these interferons fish, we examined effects recombinant goldfish (rg) IFNγrel on macrophage antimicrobial responses, immune gene expression, their signaling pathways. Our findings indicate that rgIFNγ rgIFNγrel possess unique capacities to mediate each above processes. Q-PCR analysis revealed similar expression both cytokines in tissues cell populations goldfish, although mRNA levels were generally higher most types. Whereas had long-lasting priming monocyte ROI production, relatively short-lived potential eventually down-regulated production induced by or rgTNFα2. modest phagocytic nitric oxide responses macrophages, significantly phagocytosis, iNOSA iNOSB compared with rgIFNγ. The different profiles monocytes. These differences included induction TNFα2, CXCL8, ceruloplasmin, interferon regulatory factor (IRFs) after activation monocytes rgIFNγrel. was more abundant whole lysates Both phosphorylation Stat1, while nuclear localization Stat1 only observed following treatment suggest presence functional segregation fish.