Raman spectroscopic detection of Nickel impact on single Streptomyces cells – possible bioindicators for heavy metal contamination

摘要: Heavy metal contamination of soil has an immense impact on the surrounding environment, such as ground water, and hence, become important issue within bioremediation. Therefore, heavy to be determined preferably cost-efficiently, rapidly, reliably. Here, bacteria genus Streptomyces are used bioindicators for investigated via micro-Raman spectroscopy. A single cell approach is studied avoid time-consuming culturing plate counting. Bacteria galilaeus were incubated in Ni2+ enriched media spectra recorded. Supervised statistics linear discriminant analysis was evaluate influence culture age anion bacterial cells, which been minor compared with spectral Ni2+. The identification Raman according different concentration ranges accomplished a prediction accuracy about 88%. we conclude that can bioindicator predict concentrations micromolar range. Copyright © 2011 John Wiley & Sons, Ltd.