Modulation of hippocampal acetylcholine release: a potent central action of interleukin-2

摘要: The potential of the T-cell growth factor interleukin-2 (IL-2) to modulate release ACh from rat hippocampus was studied in vitro, as a means investigate possible functional significance this cytokine CNS. Hippocampal slices were superfused with Krebs' buffer medium, and endogenous released into superfusate measured using radioenzymatic assay. Recombinant human IL-2 present during stimulation 25 mM KCl altered, concentration- dependent manner, evoked transmitter release. At concentration 15 U/ml (< or = 1 nM), inhibited by more than 50% control level (evoked untreated contralateral hemispheres). Inhibition observed within 20 min tissue exposure lasted for up hr. inhibitory effect reversible since transient did not affect subsequent at also significantly decreased frontal cortical slices, but ineffective parietal cortex striatum, revealing that selectively modulates certain, all, cholinergic nerve terminals very low concentrations (1.5 mU/ml, < 0.1 pM), transiently increased hippocampal release, resulting biphasic dose-response profile no significant 0.015 mU/ml fM). Other cytokines (IL-1 alpha, IL-3, IL-5, IL-6, interferon alpha), tested slice incubations, failed release.(ABSTRACT TRUNCATED AT 250 WORDS)