Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.

摘要: The SNARE proteins are essential components of the intracellular fusion machinery. It is thought that they form a tight four-helix complex between membranes, in effect initiating fusion. Most SNAREs contain single coiled-coil region, referred to as motif, directly adjacent transmembrane domain. neuronal SNAP-25 defines subfamily with two helices connected by longer linker, comprising also SNAP-23 and SNAP-29. We now report initial characterization novel vertebrate homologue termed SNAP-47. Northern blot immunoblot analysis revealed ubiquitous tissue distribution, particularly high levels nervous tissue. In neurons, SNAP-47 shows widespread distribution on membranes enriched synaptic vesicle fractions. vitro, substituted for formation syntaxin 1a synaptobrevin 2, it proteoliposome However, neither assembly nor was efficient SNAP-25.