Translation of the Prion Protein mRNA Is Robust in Astrocytes but Does Not Amplify during Reactive Astrocytosis in the Mouse Brain
作者： Walker S. Jackson , Clemens Krost , Andrew W. Borkowski , Lech Kaczmarczyk
DOI: 10.1371/JOURNAL.PONE.0095958
关键词:
摘要: Prion diseases induce neurodegeneration in specific brain areas for undetermined reasons. A thorough understanding of the localization disease-causing molecule, prion protein (PrP), could inform on this issue but previous studies have generated conflicting conclusions. One more intriguing disagreements is whether PrP synthesized by astrocytes. We developed a knock-in reporter mouse line which coding sequence expressing gene (Prnp), was replaced with that green fluorescent (GFP). Native GFP fluorescence intensity varied between and within regions. present astrocytes did not increase during reactive gliosis induced scrapie infection. Therefore, associated does cause an acceleration local production. In addition to aiding Prnp activity studies, will likely prove useful analysis chimeric animals produced stem cell tissue transplantation experiments.
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