Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.
作者： J.S. Crowe , H.J. Cooper , M.A. Smith , M.J. Sims , D. Parker
DOI: 10.1093/NAR/19.1.184
关键词:
摘要: … the Taq polymerase remains bound to the DNA and therefore inhibits restriction endonuclease activity, we incorporated a proteinase K digestion step prior to endonuclease digestion …
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Alan R. Shuldiner, Laurie A. Scott, Jesse Roth, PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products Nucleic Acids Research. ,vol. 18, pp. 1920- 1920 ,(1990) , 10.1093/NAR/18.7.1920