Genetic Diversity ofDatisca cannabina-Compatible Frankia Strains asDetermined bySequence Analysis ofthe PCR-Amplified 16SrRNAGene

作者： M. Sajjadmirza , Antoon D. L. Akkermans

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摘要: ThepresenceofFrankia strains insoil samples collected fromnorthern areasofPakistan was detected by inoculating Coriaria nepaknsis andDatisca cannabina plants. Theabundance ofcompatible Frankia in some areaswas indicated byprofuse nodulation ofthehostplants, whereas soil fromotherlocalities failed toresult innodulation. Anoligonucleotide probe(COR/DAT) directed against the16SrRNAgene ofthe endophytes ofCoriaria spp.thatdidnotcross-react withtheRNA geneofFrankia isolated fromotherhosts developed. Genetic diversity among nodulating D.cannabina determined bysequenceanalysis ofthepartial 16SrRNAgeneamplified fromnodules induced bysoil fromdifferent localities byPCR.FourtypesofFrankia sequencesandone non-Frankia sequence were byhybridization withaFrankia genus probeandtheCORIDATprobe aswell asbysequenceanalysis cloned PCRproducts. Nitrogen-fixing rootnodules byFrankia actinomyceteshavebeenreported formore than200nonleguminous plantspecies (4).Afterthefirst successful isolation ofa strain fromComptonia peregrina(10), hundreds of isolates havebeenpurified actinorhizal nodules. Withtheexception ofone attempt(3), all obtained fromnodules, wheretheendophyte isfoundin greaterrelative abundance andpurity thanitisinsoil. As research on spp. remained focused mainly its biology inpure culture or inrootnodules, relatively little published information abouttheasymbiotic stageor extranodular survival oftheendophyte isavailable (7,20,27, 41,43,44,47,54).Thesestudies confirmed theexistence viable cells bysuccessful ofthehost Practical application ofactinorhizal plant species (14, 50)inforestry, soilerosion control, andsoilimprovement projects requires further populations soil.

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Genetic Diversity ofDatisca cannabina-Compatible Frankia Strains asDetermined bySequence Analysis ofthe PCR-Amplified 16SrRNAGene

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Genetic Diversity ofDatisca cannabina-Compatible Frankia Strains asDetermined bySequence Analysis ofthe PCR-Amplified 16SrRNAGene

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