Restriction analysis of the Frankia spp. genome
作者： Svetlana V. Dobritsa
DOI: 10.1111/J.1574-6968.1985.TB00846.X
关键词: Endonuclease 、 Elaeagnus 、 Genotype 、 Colletia 、 Frankia 、 Biology 、 Restriction enzyme 、 Microbiology 、 Genetics 、 Gel electrophoresis 、 Agarose gel electrophoresis
摘要: Total cellular DNAs of 10 Frankia isolates from Alnus, Elaeagnus and Colletia spp. root nodules were cleaved with ten site-specific restriction endonucleases analysed by agarose gel electrophoresis. Among the tested, Bam HI, Bgl II, Sal I Sma proved to be most suitable for genome analysis in DNA banding patterns reproducible characteristic each strain. The different strains differed marked indicating considerable genotypic heterogeneity among isolates. approach can used strain identification as well differentiation between phenotypically similar strains.
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