A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

摘要: Art v 1, the major pollen allergen of composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with bulky arabinogalactan-protein moiety. A close relative mugwort, ragweed (Ambrosia artemisiifolia) is an important source in North America, and, since 1990, become growing health concern Europe well. Weed pollen-sensitized patients demonstrated IgE reactivity to apparently 29–31 kDa. This reaction could be inhibited by 1. The purified consisted 57-amino acid-long defensin-like domain high homology 1 and C-terminal proline-rich domain. part contained hydroxyproline-linked arabinogalactan chains one galactose 5 20 more α-arabinofuranosyl residues some β-arabinoses terminal positions revealed field NMR. only small amounts single β-arabinosyl residues, which form binding determinant cDNA clones for this were obtained from flowers. Immunological characterization that recombinant reacted >30% weed allergic patients. Therefore, constitutes novel designated Amb 4.